Fluorochrome

Browse By Fluorochrome

 

Compare filter sets by selecting the recommended/alternative set listed for each fluorochrome below.

  • If you need more information, please click fluorochrome. You can see each fluorochrome information and recommended filter sets.
  • If you need other dye information, please contact us.
  • Tel : 02-3473-4188~9
  • E-mail : jhjin@jnoptic.com

 

 

 

AlexaFluor 594

Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 dye is pH-insensitive over a wide molar range.

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CFP

Cyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm.

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Cy5

Cy5 dye is a bright, far-red-fluorescent dye that can be excited using the 633 or 647 nm laser lines, and visualized Cy5 filter sets.  In addition to immunocytochemistry applications, Cy5 is also commonly used to label nucleic acids.

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Cy5.5

A variety of cyanine 5.5 (Cy5.5) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5 dyes are one type of the most common red fluorophores.

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Cy7

Cy7 is a near-IR fluor that is invisible to the naked eye (Excitation/emission maximum 750/776 nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye.

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DAPI

DAPI (4′,6-diamidino-2-phenylindole) is a fluorescent stain that binds strongly to A-T rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.

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Ethidium Bromide

As with most fluorescent compounds, ethidium bromide is aromatic. Its core heterocyclic moiety is generically known as a phenanthridine, an isomer of which is the fluorescent dye acridine. Absorption maxima of EtBr in aqueous solution are at 210 nm and 285 nm, which correspond to ultraviolet light. As a result of this excitation, EtBr emits orange light with wavelength 605 nm.

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FITC

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluoresce in molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.

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GFP

The green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.

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tdTomato

tdTomato is a genetic fusion of two copies of the dTomato gene (2) which was specifi cally designed for low aggregation (1). Its tandem dimer structure plays an important role in the exceptional brightness of tdTomato (Table I). Its excitation and emission maxima occur at 554 nm and 581 nm, respectively (1). Because tdTomato forms an intramolecular dimer, it behaves like a monomer, and has been used successfully for N- and C- terminal fusions. It shows excellent photostability and its half-time (t0.5) for maturation is one hour at 37°C.

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TRITC

Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.

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Fluorescence Illumination

형광 관찰 조명장치의 종류

 

  • Mercury : 형광 현미경법에 가장 흔히 사용하는 광원으로, 자외선 및 가시광선 영역에서 아주 밝다.

Spectrum of mercury

 

  • Xenon : 가시광선에서 상대적으로 연속적인 스펙트럼을 가진다. 염료 및/또는 시료의 스펙트럼 특성을 정량적으로 분석하는 시스템에서는 제논 램프를 사용하는 것이 좋지만, 같은 와트수의 수은 램프만큼 밝지는 않다.
Spectrum of Xenon

 

  • LED :  약 10 ~ 40nm 범위의 상대적으로 좁은 스펙트럼 대역폭 내에서 빛을 방출한다는 고유의 특징이 있다. 일반적으로 정격 수명이 10,000시간 이상이고 50,000시간 이상 사용하는 경우도 많고, 전체 정격 수명에 걸쳐 출력 강도가 비교적 일정하게 유지된다.

    Spectrum of LED

     

  • Laser : 보통은 단색광의 좁은 스펙트럼 대역에서 극히 밝다. 하지만 레이저광의 간섭성으로 인해 샘플 영역에 균일한 조도로 비추기 어렵기 때문에 형광 현미경 검사에는 레이저가 거의 사용되지 않는다.

    Spectrum of LASER

 

  • Metal-halide : 유효수명이 최대 2000시간으로, 일반적인 수은 램프의 200시간이나 제논 램프의 400시간과 비교하면 훨씬 길다.

 

AcquCAM 23S

AcquCAM 23S

(Down load Catalog ACQUCAM 23S)

  • USB 3.0 Monochrome Camera
  • 1/1.2 ” Exmor Sensor
  • 1920×1200 pixel
  • Trigger input and I/O

AcquCAM 23S
EXMOR Sensors: Next-generation imaging sensors based on CMOS, increase the strength and reduce noise.

 

High sensitivity camera available from visible to IR range

20151120_1(CA1 pyramidal neuron)
Apply high sensitivity EXMOR sensor with additional filter block visible range, IR Cut filter image could be observed.

 

Available wider range of observation

AcquCAM 23S_6
By applying a 1 / 1.2 ” EXMOR sensor, you can observe a wider range of superior sensitivity image.
These images were shot by AcquCAM 23S Package.

High sensitivity and fast frame image

AcquCAM 23S_(CA3 pyramidal neuron)

AcquCAM 23S_(Dentate gyrus Granule cell)
To maximize the range of imaging possible observation with fast frame 54Fps and high sensitivity 0.015 lx .

[Specification]

Spec Description
Size 1/1.2 inch
Resolution H: 1920, V: 1200
Pixel size H: 5.86 µm, V: 5.86 µm
Video formats

& Frame rate

1920×1200 Y16 @ 25 fps

1920×1200 Y800 @ 54 fps

Sensitivity 0.015 lx
Exposure time 1/100000 to 30 s
Lens mount C/Cs mount
Interface USB 3.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass 65 g

 

AcquCAM Pro/S3

AcquCAM Pro/S3

(Down load Catalog_ACQUCAM PRO/S3)

AcquCAM Pro/S3

AcquCAM Pro/S3 USB 3.0 camera is the perfect solution for many bio, industrial, medical applications. Utilizing the very sensitive mono CCD sensors, these cameras feature a specialization of fluorescence observations. With up to 30 fps and a trigger delay of less than 5 micro seconds.

  • 1.2 Mega pixel CCDcamera
  • Monochrome, Progressive scan
  • Preview software provided (JNOPTIC Capture)
  • Software compatible with Windows XP/ Vista/ 7/ 8 operating systems

 

Apply Pseudo color for live fluorescence

 

Apply Pseudo color for black live fluorescence

AcquCAM Pro S3_6

 

Emphasis function for variety images

 

Getting clear image with general light source

 

[Specification]
Spec Description
Size 1/3 inch
Resolution H: 1280, V: 960
Pixel size H: 3.75 µm, V: 3.75 µm
Video formats

& Frame rate

1280×960 Y800 @ 30, 20, 15, 7.5, 3.75 fps

1280×960 Y16 @ 30, 20, 15, 7.5, 3.75 fps

Sensitivity 0.015 lx
Exposure time 1/10000 to 30 s
Lens mount C/Cs mount
Interface USB 3.0
Power supply 4.5 to 5.5 VDC
Dimensions H: 29 mm, W: 29 mm, L: 43 mm
Mass 65 g